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human lung microvascular endothelial cells  (ATCC)


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    ATCC human lung microvascular endothelial cells
    Human Lung Microvascular Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+microvascular+endothelial+cells/HULEC-5a/pm42082453-365-16-35
    Average 96 stars, based on 160 article reviews
    human lung microvascular endothelial cells - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Modeling Endothelial Dysfunction in IPF: Bridging Mechanistic Insights and Translational Applications
    Article Snippet: Human lung microvascular endothelial cells (HULEC-5a, CRL-3244) were obtained from ATCC (Manassas, USA).

    Article Title: Octreotide Counteracts IFN‐γ‐Induced Endothelial Inflammation
    Article Snippet: Human lung microvascular endothelial cells (HULEC‐5a) were maintained in endothelial cell growth medium (PCS‐100‐030) supplemented with a specialized endothelial cell growth kit (PCS‐100‐040) purchased from ATCC (Manassas, VA).

    Article Title: STUB1-induced polyubiquitination of SIK3 in alveolar type 2 epithelial cells alleviates severity and outcomes of acute lung injury.
    Article Snippet: The human lung adenocarcinoma A549 cells (Cat.#: CRM-CCL-185), human pulmonary artery endothelial cells (HPAEC, Cat.#: PCS-100-022), human lung microvascular endothelial cells (HULEC-5a) and human umbilical vein endothelial cell (HUVEC, Cat.#: PCS-100-013) were all obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

    Cell Culture:

    Article Title: Activation of USP30 Disrupts Endothelial Cell Function and Aggravates Acute Lung Injury Through Regulating the S-Adenosylmethionine Cycle.
    Article Snippet: .. Cells and Reagents: Human lung microvascular endothelial cells (HLMVECs, from ATCC) were cultured with endothelial growth medium2 (EGM-2) at 37 °C in a cell culture incubator with 5% CO2. .. HEK293/TLR4/MD2 cells (from InvivoGen) were cultured in DMEM medium containing 10% fetal bovine serum (FBS).

    Article Title: USP19 alleviates LPS-induced acute lung injury via inhibiting TAK1 activation
    Article Snippet: Detection employed HRP-conjugated secondary antibodies (PV-9001/PV-9004, ZSGB-BIO) with DAB chromogen (ZLI-9018, ZSGB-BIO). .. Human lung microvascular endothelial cells (HULEC-5a; ATCC CRL-3244) were cultured in MCDB131 medium supplemented with 10% FBS (Newzeru), 10 ng/mL EGF, 1 μg/mL hydrocortisone, 10 mM L-glutamine (Beyotime), and 1% penicillin-streptomycin (Biosharp) at 37 °C with 5% CO2. .. For LPS challenge, cells were treated with 100 ng/mL LPS (Beyotime) for 24 h.

    Article Title: USP19 alleviates LPS-induced acute lung injury via inhibiting TAK1 activation.
    Article Snippet: .. Human lung microvascular endothelial cells (HULEC-5a; ATCC CRL-3244) were cultured in MCDB131 medium supplemented with 10% FBS (Newzeru), 10 ng/mL EGF, 1 μg/mL hydrocortisone, 10 mM L-glutamine (Beyotime), and 1% penicillin-streptomycin (Biosharp) at 37°C with 5% CO2. ..

    Article Title: Donor Sex and Platelet Storage Influence the Therapeutic Effects of Platelet-Derived Extracellular Vesicles on Endothelial Barrier Function
    Article Snippet: PEVs were absorbed onto glow-discharged, carbon-coated copper grids (200 mesh; Agar Scientific, UK) for 60 s, dried, and negatively stained with 3% uranyl acetate (System Biosciences, USA) for 60 s. Imaging was performed on a FEI Tecnai 12 TEM at 120 kV with an AMT XR111 CCD camera at the Thomas Jefferson University Core Facility. .. Human lung microvascular endothelial cells (HULEC-5a, CRL-3244; ATCC) were cultured in MCDB131 basal medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin – streptomycin, 10 mM glutamine, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. ..

    Article Title: Ceapin-A7 counteracts the protective effects of Lanreotide in endothelial cells
    Article Snippet: Corning trans-well cell culture inserts (CLS3470), β-actin antibody (A5441), Ceapin-A7 (SML2330-5 MG), and FITC-Dextran (46945) were purchased from Sigma-Aldrich (St. Louis, MO). .. Human lung microvascular endothelial cells (HULEC-5a) were cultured using endothelial cell growth medium (PCS-100-030) enriched with a growth factor supplement kit (PCS-100-040), sourced from ATCC (Manassas, VA). .. Bovine pulmonary artery endothelial cells (BPAEC) were obtained from Genlantis (San Diego, CA) and cultured in DMEM (VWRL0101–0500) supplemented with 10% fetal bovine serum (VWR, Radnor, PA).



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    (A) Schematic overview of the human lung cell lines used in the study. (B–F) PUUV infection kinetics over five days, quantified as the percentage of infected cells by immunofluorescence staining for PUUV nucleocapsid protein with nuclear counterstaining in (B) A549 alveolar epithelial cells, (C) BEAS-2B bronchial epithelial cells, <t>(D)</t> <t>HULEC-5a</t> microvascular lung endothelial cells, (E) MRC-5 lung fibroblasts, and (F) A549/PIV5-V cells with impaired STAT1 signaling. Statistical significance was calculated by unpaired t-test (*P < 0.05, **P < 0.01; ns, not significant). (G–K) HA levels in culture media supernatants collected at the indicated time points from PUUV-infected (G) A549, (H) BEAS-2B, (I) HULEC-5a, (J) MRC-5, and (K) A549/PIV5-V cells. HA concentrations were measured by ELISA and are shown as percentage of mock-infected controls. Data represents three independent experiments with duplicate technical replicates per experiment. Bars show mean ± SEM. Statistical significance was assessed using multiple unpaired t-tests with Holm–Šídák correction for multiple comparisons (*P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant).
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    (A) Schematic overview of the human lung cell lines used in the study. (B–F) PUUV infection kinetics over five days, quantified as the percentage of infected cells by immunofluorescence staining for PUUV nucleocapsid protein with nuclear counterstaining in (B) A549 alveolar epithelial cells, (C) BEAS-2B bronchial epithelial cells, <t>(D)</t> <t>HULEC-5a</t> microvascular lung endothelial cells, (E) MRC-5 lung fibroblasts, and (F) A549/PIV5-V cells with impaired STAT1 signaling. Statistical significance was calculated by unpaired t-test (*P < 0.05, **P < 0.01; ns, not significant). (G–K) HA levels in culture media supernatants collected at the indicated time points from PUUV-infected (G) A549, (H) BEAS-2B, (I) HULEC-5a, (J) MRC-5, and (K) A549/PIV5-V cells. HA concentrations were measured by ELISA and are shown as percentage of mock-infected controls. Data represents three independent experiments with duplicate technical replicates per experiment. Bars show mean ± SEM. Statistical significance was assessed using multiple unpaired t-tests with Holm–Šídák correction for multiple comparisons (*P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant).
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    Image Search Results


    (A) Schematic overview of the human lung cell lines used in the study. (B–F) PUUV infection kinetics over five days, quantified as the percentage of infected cells by immunofluorescence staining for PUUV nucleocapsid protein with nuclear counterstaining in (B) A549 alveolar epithelial cells, (C) BEAS-2B bronchial epithelial cells, (D) HULEC-5a microvascular lung endothelial cells, (E) MRC-5 lung fibroblasts, and (F) A549/PIV5-V cells with impaired STAT1 signaling. Statistical significance was calculated by unpaired t-test (*P < 0.05, **P < 0.01; ns, not significant). (G–K) HA levels in culture media supernatants collected at the indicated time points from PUUV-infected (G) A549, (H) BEAS-2B, (I) HULEC-5a, (J) MRC-5, and (K) A549/PIV5-V cells. HA concentrations were measured by ELISA and are shown as percentage of mock-infected controls. Data represents three independent experiments with duplicate technical replicates per experiment. Bars show mean ± SEM. Statistical significance was assessed using multiple unpaired t-tests with Holm–Šídák correction for multiple comparisons (*P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant).

    Journal: medRxiv

    Article Title: Puumala orthohantavirus dysregulates hyaluronan metabolism in lung cells and correlates with disease severity and lung impairment

    doi: 10.64898/2026.03.10.26348053

    Figure Lengend Snippet: (A) Schematic overview of the human lung cell lines used in the study. (B–F) PUUV infection kinetics over five days, quantified as the percentage of infected cells by immunofluorescence staining for PUUV nucleocapsid protein with nuclear counterstaining in (B) A549 alveolar epithelial cells, (C) BEAS-2B bronchial epithelial cells, (D) HULEC-5a microvascular lung endothelial cells, (E) MRC-5 lung fibroblasts, and (F) A549/PIV5-V cells with impaired STAT1 signaling. Statistical significance was calculated by unpaired t-test (*P < 0.05, **P < 0.01; ns, not significant). (G–K) HA levels in culture media supernatants collected at the indicated time points from PUUV-infected (G) A549, (H) BEAS-2B, (I) HULEC-5a, (J) MRC-5, and (K) A549/PIV5-V cells. HA concentrations were measured by ELISA and are shown as percentage of mock-infected controls. Data represents three independent experiments with duplicate technical replicates per experiment. Bars show mean ± SEM. Statistical significance was assessed using multiple unpaired t-tests with Holm–Šídák correction for multiple comparisons (*P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant).

    Article Snippet: Human lung microvascular endothelial cells HULEC-5a (CRL-3244TM, American Type Culture Collection, Manassas, VA, USA) were cultured in MCDB 131 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10 ng/mL human epidermal growth factor (Sigma-Aldrich, St. Louis, MO, USA), 1 μg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 100 U/mL Penicillin-Streptomycin and 10% FBS.

    Techniques: Infection, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay

    (A) Schematic overview of genes involved in HA synthesis ( HAS1–3 ), HA degradation ( HYAL1–2 ), and HA signaling ( CD44 ). (B–F) Expression of HA-regulating genes in (B) A549, (C) BEAS-2B, (D) HULEC-5a, (E) MRC-5, and (F) A549/PIV5-V cells, determined by quantitative PCR at the indicated time points. Expression levels were normalized to β-actin and are shown as fold change relative to uninfected controls. Data represents three independent experiments with duplicate technical replicates per experiment. Results are displayed as box-and-whisker plots, where the box indicates the 25th to 75th percentiles (interquartile range), the line within the box represents the median, and the whiskers denote the minimum and maximum values. Statistical significance was assessed using paired t-tests, comparing the expression values to the respective time-matched uninfected controls (*P < 0.05, **P < 0.01, ***P < 0.001).

    Journal: medRxiv

    Article Title: Puumala orthohantavirus dysregulates hyaluronan metabolism in lung cells and correlates with disease severity and lung impairment

    doi: 10.64898/2026.03.10.26348053

    Figure Lengend Snippet: (A) Schematic overview of genes involved in HA synthesis ( HAS1–3 ), HA degradation ( HYAL1–2 ), and HA signaling ( CD44 ). (B–F) Expression of HA-regulating genes in (B) A549, (C) BEAS-2B, (D) HULEC-5a, (E) MRC-5, and (F) A549/PIV5-V cells, determined by quantitative PCR at the indicated time points. Expression levels were normalized to β-actin and are shown as fold change relative to uninfected controls. Data represents three independent experiments with duplicate technical replicates per experiment. Results are displayed as box-and-whisker plots, where the box indicates the 25th to 75th percentiles (interquartile range), the line within the box represents the median, and the whiskers denote the minimum and maximum values. Statistical significance was assessed using paired t-tests, comparing the expression values to the respective time-matched uninfected controls (*P < 0.05, **P < 0.01, ***P < 0.001).

    Article Snippet: Human lung microvascular endothelial cells HULEC-5a (CRL-3244TM, American Type Culture Collection, Manassas, VA, USA) were cultured in MCDB 131 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10 ng/mL human epidermal growth factor (Sigma-Aldrich, St. Louis, MO, USA), 1 μg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 100 U/mL Penicillin-Streptomycin and 10% FBS.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Whisker Assay